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preparation kit  (Illumina Inc)


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    Structured Review

    Illumina Inc preparation kit
    Preparation Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 11826 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/xt+dna+library+preparation+kit/Nextera+XT+DNA+Library+Preparation+Kit/pmc12848573-228-17-20
    Average 99 stars, based on 11826 article reviews
    preparation kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Construct:

    Article Title: Emergence of tmexCD1-toprJ1 in the clinical high-risk clone sequence type 147 among carbapenem-resistant Klebsiella pneumoniae isolates in China: A multicentre retrospective study.
    Article Snippet: Genomic DNA was extracted using a Bacterial DNA Kit (Omega BioTek, USA). .. The genomic DNA libraries were constructed using the Nextera XT DNA Library Preparation Kit (Illumina, San Diego, CA, USA) following the manufacturer’s standard protocols. .. The libraries for Nanopore sequencing were prepared using the SQK-RBK004 Rapid Barcoding Kit (Oxford Nanopore Technologies, UK).

    Article Title: Emergence of tmexCD1-toprJ1 in the clinical high-risk clone sequence type 147 among carbapenem–resistant Klebsiella pneumoniae isolates in China: A multicentre retrospective study
    Article Snippet: Genomic DNA was extracted using a Bacterial DNA Kit (Omega BioTek, USA). .. The genomic DNA libraries were constructed using the Nextera XT DNA Library Preparation Kit (Illumina, San Diego, CA, USA) following the manufacturer’s standard protocols. .. The libraries for Nanopore sequencing were prepared using the SQK-RBK004 Rapid Barcoding Kit (Oxford Nanopore Technologies, UK).

    DNA Library Preparation:

    Article Title: Emergence of tmexCD1-toprJ1 in the clinical high-risk clone sequence type 147 among carbapenem-resistant Klebsiella pneumoniae isolates in China: A multicentre retrospective study.
    Article Snippet: Genomic DNA was extracted using a Bacterial DNA Kit (Omega BioTek, USA). .. The genomic DNA libraries were constructed using the Nextera XT DNA Library Preparation Kit (Illumina, San Diego, CA, USA) following the manufacturer’s standard protocols. .. The libraries for Nanopore sequencing were prepared using the SQK-RBK004 Rapid Barcoding Kit (Oxford Nanopore Technologies, UK).

    Article Title: Emergence of tmexCD1-toprJ1 in the clinical high-risk clone sequence type 147 among carbapenem–resistant Klebsiella pneumoniae isolates in China: A multicentre retrospective study
    Article Snippet: Genomic DNA was extracted using a Bacterial DNA Kit (Omega BioTek, USA). .. The genomic DNA libraries were constructed using the Nextera XT DNA Library Preparation Kit (Illumina, San Diego, CA, USA) following the manufacturer’s standard protocols. .. The libraries for Nanopore sequencing were prepared using the SQK-RBK004 Rapid Barcoding Kit (Oxford Nanopore Technologies, UK).

    Article Title: Metagenomic surveillance of tick-borne pathogens and microbiomes in Huntingdon County, Pennsylvania
    Article Snippet: Concentrated extracts were again quantified using the Qubit Flex Fluorometer and 1× Qubit dsDNA High Sensitivity Assay Kit (Thermo Fisher Scientific, Waltham, Massachusetts). .. Samples that tested positive for at least one pathogen in PCR were selected for metagenomics (33 of the 96 samples), where their quantified DNA extracts were used as normalized input for the Nextera XT DNA Library Preparation Kit (Illumina, San Diego, CA). .. Libraries were quality checked using an Agilent 2100 Bioanalyzer and DNA High Sensitivity kit (Agilent Technologies, Santa Clara, CA) and then pooled in an equimolar ratio.

    Article Title: Antibiotic susceptibility and resistance genes in Escherichia coli from broilers reared in a low-antibiotic-use production system
    Article Snippet: The DNA concentration was measured using the Qubit dsDNA High Sensitivity Assay Kit on a Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA). .. Library preparation was performed using the Nextera XT DNA Library Preparation Kit (Illumina, San Diego, CA, USA), and library quality was assessed with the High Sensitivity DNA ScreenTape D1000 on the 4150 TapeStation System (Agilent Technologies, Santa Clara, CA, USA). .. Prepared DNA libraries were sent to SciLifeLab Clinical Genomics (Solna, Stockholm) for sequencing on an Illumina NovaSeq X instrument, generating 2 × 150 bp reads.

    Article Title: A WGS workflow for identifying genetically modified and foodborne-pathogenic Bacillus isolates
    Article Snippet: For the wt B. velezensis samples and the B. cereus s.l. datasets (excluding the B. cereus s.s. samples obtained from another study), DNA from bacterial isolates was extracted using Quick-DNATM HMW MagBead Kit (Zymo Research, Irvine, CA, USA) according to the manufacturer's instructions and then sequenced on the Illumina platform, following previously described protocol ( ). .. Short-read DNA libraries were prepared using the Nextera XT DNA library preparation kit (Illumina, San Diego, CA, USA) according to manufacturer's instructions. .. Sequencing was carried out on an Illumina MiSeq system with the V3 chemistry, obtaining 250 bp paired-end reads, and aiming for a theoretical coverage of 60× per sample, based on an average Bacillus genome size of 4.2 Mbp.

    Article Title: A human-associated Spiroplasma ixodetis lineage responsible for infantile cataracts and adult febrile illness
    Article Snippet: .. Spiroplasma MAGs were obtained by sequencing DNA extracts from infected patient samples ( ) using the Illumina HiSeq 2500 technology and after a library preparation performed with Nextera XT DNA Library Preparation Kit (Illumina, USA). ..

    Article Title: Irisin ameliorates obesity and insulin-resistance via adipose-tissue IL-33 and regulatory T cells
    Article Snippet: .. The Nextera XT DNA Library Preparation Kit (Illumina) was used for Tn5 transposon-based fragmentation followed by PCR amplification for an additional 12 cycles using barcoded primers such that each sample carried a specific combination of Illumina P5 and P7 barcodes. .. Paired-end sequencing was performed on an Illumina NextSeq500 (two full NextSeq runs per plate for an average of 10M raw reads per sample) using two 38-bp reads with no further trimming.

    Article Title: Irisin ameliorates obesity and insulin resistance via adipose tissue IL-33 and regulatory T cells.
    Article Snippet: .. The Nextera XT DNA Library Preparation Kit (Illumina) was used for Tn5 transposon-based fragmentation followed by PCR amplification for an additional 12 cycles using barcoded primers such that each sample carried a specific combination of Illumina P5 and P7 barcodes. .. Paired-end sequencing was performed on an Illumina NextSeq 500 (two full NextSeq runs per plate for an average of 10 M raw reads per sample) using two 38-base-pair reads with no further trimming.

    Polymerase Chain Reaction:

    Article Title: Metagenomic surveillance of tick-borne pathogens and microbiomes in Huntingdon County, Pennsylvania
    Article Snippet: Concentrated extracts were again quantified using the Qubit Flex Fluorometer and 1× Qubit dsDNA High Sensitivity Assay Kit (Thermo Fisher Scientific, Waltham, Massachusetts). .. Samples that tested positive for at least one pathogen in PCR were selected for metagenomics (33 of the 96 samples), where their quantified DNA extracts were used as normalized input for the Nextera XT DNA Library Preparation Kit (Illumina, San Diego, CA). .. Libraries were quality checked using an Agilent 2100 Bioanalyzer and DNA High Sensitivity kit (Agilent Technologies, Santa Clara, CA) and then pooled in an equimolar ratio.

    Article Title: Irisin ameliorates obesity and insulin-resistance via adipose-tissue IL-33 and regulatory T cells
    Article Snippet: .. The Nextera XT DNA Library Preparation Kit (Illumina) was used for Tn5 transposon-based fragmentation followed by PCR amplification for an additional 12 cycles using barcoded primers such that each sample carried a specific combination of Illumina P5 and P7 barcodes. .. Paired-end sequencing was performed on an Illumina NextSeq500 (two full NextSeq runs per plate for an average of 10M raw reads per sample) using two 38-bp reads with no further trimming.

    Article Title: Irisin ameliorates obesity and insulin resistance via adipose tissue IL-33 and regulatory T cells.
    Article Snippet: .. The Nextera XT DNA Library Preparation Kit (Illumina) was used for Tn5 transposon-based fragmentation followed by PCR amplification for an additional 12 cycles using barcoded primers such that each sample carried a specific combination of Illumina P5 and P7 barcodes. .. Paired-end sequencing was performed on an Illumina NextSeq 500 (two full NextSeq runs per plate for an average of 10 M raw reads per sample) using two 38-base-pair reads with no further trimming.

    Metagenomics:

    Article Title: Metagenomic surveillance of tick-borne pathogens and microbiomes in Huntingdon County, Pennsylvania
    Article Snippet: Concentrated extracts were again quantified using the Qubit Flex Fluorometer and 1× Qubit dsDNA High Sensitivity Assay Kit (Thermo Fisher Scientific, Waltham, Massachusetts). .. Samples that tested positive for at least one pathogen in PCR were selected for metagenomics (33 of the 96 samples), where their quantified DNA extracts were used as normalized input for the Nextera XT DNA Library Preparation Kit (Illumina, San Diego, CA). .. Libraries were quality checked using an Agilent 2100 Bioanalyzer and DNA High Sensitivity kit (Agilent Technologies, Santa Clara, CA) and then pooled in an equimolar ratio.

    Sequencing:

    Article Title: A human-associated Spiroplasma ixodetis lineage responsible for infantile cataracts and adult febrile illness
    Article Snippet: .. Spiroplasma MAGs were obtained by sequencing DNA extracts from infected patient samples ( ) using the Illumina HiSeq 2500 technology and after a library preparation performed with Nextera XT DNA Library Preparation Kit (Illumina, USA). ..

    Infection:

    Article Title: A human-associated Spiroplasma ixodetis lineage responsible for infantile cataracts and adult febrile illness
    Article Snippet: .. Spiroplasma MAGs were obtained by sequencing DNA extracts from infected patient samples ( ) using the Illumina HiSeq 2500 technology and after a library preparation performed with Nextera XT DNA Library Preparation Kit (Illumina, USA). ..

    Amplification:

    Article Title: Irisin ameliorates obesity and insulin-resistance via adipose-tissue IL-33 and regulatory T cells
    Article Snippet: .. The Nextera XT DNA Library Preparation Kit (Illumina) was used for Tn5 transposon-based fragmentation followed by PCR amplification for an additional 12 cycles using barcoded primers such that each sample carried a specific combination of Illumina P5 and P7 barcodes. .. Paired-end sequencing was performed on an Illumina NextSeq500 (two full NextSeq runs per plate for an average of 10M raw reads per sample) using two 38-bp reads with no further trimming.

    Article Title: Irisin ameliorates obesity and insulin resistance via adipose tissue IL-33 and regulatory T cells.
    Article Snippet: .. The Nextera XT DNA Library Preparation Kit (Illumina) was used for Tn5 transposon-based fragmentation followed by PCR amplification for an additional 12 cycles using barcoded primers such that each sample carried a specific combination of Illumina P5 and P7 barcodes. .. Paired-end sequencing was performed on an Illumina NextSeq 500 (two full NextSeq runs per plate for an average of 10 M raw reads per sample) using two 38-base-pair reads with no further trimming.



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